Hotspot

psaC

SSC · 125749-127621 · 1873 bp

Back to Oedogonium

Pi 0.167

MarkerSeek score 82.10

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 1873
variable_sites: 488
pi: 0.167
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	TGGTTCGAAGCCAGAAACTC	GGGACTGATTTACCACCAGG	50.0	55.0	58.1	57.6	2788-2825 bp	0.5	58.3
psaC_p2	ATGGGTGCGACTCGTTTAAA	GGGACTGATTTACCACCAGG	45.0	55.0	57.8	57.6	2942-2979 bp	0.5	58.2
psaC_p3	TGGGTGCGACTCGTTTAAAT	GGGACTGATTTACCACCAGG	45.0	55.0	57.8	57.6	2941-2978 bp	0.5	58.2
psaC_p4	GGTTCGAAGCCAGAAACTCT	GGGACTGATTTACCACCAGG	50.0	55.0	57.8	57.6	2787-2824 bp	0.5	57.9
psaC_p5	AGTATATGGGTGCGACTCGT	GGGACTGATTTACCACCAGG	50.0	55.0	58.3	57.6	2947-2984 bp	0.5	57.1