Hotspot

orf458

LSC · 60573-61949 · 1377 bp

Back to Oedogonium

Pi 0.21

MarkerSeek score 83.70

Primer available yes

Label orf458

Feature Metadata

label_name: orf458
feature_type: gene
region: LSC
length_bp: 1377
variable_sites: 459
pi: 0.21
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf458_p1	GCAGATTTTCGCAAGGCATT	AAAGCCAATGGTACCTAGCG	45.0	50.0	58.0	58.0	1782-1962 bp	0.75	81.7
orf458_p2	GCAGATTTTCGCAAGGCATT	GTACCCAATTTCAGGTGGCA	45.0	50.0	58.0	58.1	2148-2325 bp	0.75	81.3
orf458_p3	GCAGATTTTCGCAAGGCATT	GAGCTGGTGGATTTTCCGAA	45.0	50.0	58.0	58.2	1647-1828 bp	0.75	80.6
orf458_p4	CCCCTAAGAACCGTACATGC	AAAGCCAATGGTACCTAGCG	55.0	50.0	58.1	58.0	1929-2005 bp	0.5	61.1
orf458_p5	CCCCTAAGAACCGTACATGC	GTACCCAATTTCAGGTGGCA	55.0	50.0	58.1	58.1	2292-2368 bp	0.5	60.7