Hotspot

orf258

IRa · 182509-183288 · 780 bp

Back to Oedogonium

Pi 0.464

MarkerSeek score 66.70

Primer available yes

Label orf258

Feature Metadata

label_name: orf258
feature_type: gene
region: IRa
length_bp: 780
variable_sites: 210
pi: 0.464
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.04
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf258_p1	AACGCAATTGAAAACCCGAC	CAATCCTTGGAGATGCCGAA	45.0	50.0	57.9	58.0	1462 bp	0.25	48.6
orf258_p2	AACGCAATTGAAAACCCGAC	CAACAATTTCCGACTGCGAC	45.0	50.0	57.9	58.0	1595 bp	0.25	48.6
orf258_p3	AACGCAATTGAAAACCCGAC	TGCCGAAGTTCTAGCTGTTC	45.0	50.0	57.9	57.9	1449 bp	0.25	48.3
orf258_p4	AACGCAATTGAAAACCCGAC	TCCGACTGCGACAATTTGAT	45.0	45.0	57.9	57.9	1587 bp	0.25	48.2
orf258_p5	AACGCAATTGAAAACCCGAC	GTGAGCACCAAATTCATCGC	45.0	50.0	57.9	58.1	1492 bp	0.25	48.2