Hotspot

orf212

IRb · 87459-88100 · 642 bp

Back to Oedogonium

Pi 0.482

MarkerSeek score 56.90

Primer available yes

Label orf212

Feature Metadata

label_name: orf212
feature_type: gene
region: IRb
length_bp: 642
variable_sites: 185
pi: 0.482
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.00
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf212_p1	TCCAATGGTCTTGGGGATCT	AAGTCGGAGTTCCTGAGACA	50.0	50.0	58.0	58.0	1227 bp	0.25	49.9
orf212_p2	TCCAATGGTCTTGGGGATCT	ACAAGTCGGAGTTCCTGAGA	50.0	50.0	58.0	58.0	1229 bp	0.25	49.9
orf212_p3	TCCAATGGTCTTGGGGATCT	AGTCGGAGTTCCTGAGACAA	50.0	50.0	58.0	58.0	1226 bp	0.25	49.9
orf212_p4	TCCAATGGTCTTGGGGATCT	ACTACGTTGAATCGCTGGAC	50.0	50.0	58.0	58.0	921 bp	0.25	49.8
orf212_p5	TCCAATGGTCTTGGGGATCT	AAGACCCTTTTGGACTGCAG	50.0	50.0	58.0	58.0	1317 bp	0.25	49.8