Hotspot

orf212-psbA

IRa · 187895-189333 · 1439 bp

Back to Oedogonium

Pi 0.395

MarkerSeek score 68.10

Primer available yes

Label orf212-psbA

Feature Metadata

label_name: orf212-psbA
feature_type: igs
region: IRa
length_bp: 1439
variable_sites: 318
pi: 0.395
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.13
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf212-psbA_p1	TGATTTGTACACGCCGGTAG	ACAACGTCAGTGTTTCTCCC	50.0	50.0	58.0	58.1	2387 bp	0.25	49.6
orf212-psbA_p2	CATCGACCGTGTCACATTCT	ACAACGTCAGTGTTTCTCCC	50.0	50.0	58.0	58.1	2236 bp	0.25	49.6
orf212-psbA_p3	TTCAACGCCAGCTATCTGAC	ACAACGTCAGTGTTTCTCCC	50.0	50.0	58.0	58.1	2605 bp	0.25	49.5
orf212-psbA_p4	CCGGTAGATTCCTACGAGGT	ACAACGTCAGTGTTTCTCCC	55.0	50.0	58.0	58.1	2374 bp	0.25	49.4
orf212-psbA_p5	ACCACAGTGGGGTAATAGCT	ACAACGTCAGTGTTTCTCCC	50.0	50.0	58.0	58.1	2328 bp	0.25	49.3