Hotspot

orf258-orf212

IRa · 183289-187252 · 3964 bp

Back to Oedogonium

Pi 0.444

MarkerSeek score 62.30

Primer available yes

Label orf258-orf212

Feature Metadata

label_name: orf258-orf212
feature_type: igs
region: IRa
length_bp: 3964
variable_sites: 884
pi: 0.444
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.05
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf258-orf212_p1	TAAAACGGCCAATTGTCCCA	CCGTTAAATCCAGCAGTCGA	45.0	50.0	58.0	58.0	925 bp	0.25	49.9
orf258-orf212_p2	TAAAACGGCCAATTGTCCCA	TCCGTTAAATCCAGCAGTCG	45.0	50.0	58.0	58.0	926 bp	0.25	49.9
orf258-orf212_p3	TAAAACGGCCAATTGTCCCA	GATGCAAAACTGATTGGGGC	45.0	50.0	58.0	58.0	490 bp	0.25	49.9
orf258-orf212_p4	TAAAACGGCCAATTGTCCCA	AGAAGGTCCGGGTGACTTTA	45.0	50.0	58.0	58.0	899 bp	0.25	49.8
orf258-orf212_p5	TAAAACGGCCAATTGTCCCA	AAAAAGAAGGTCCGGGTGAC	45.0	50.0	58.0	58.0	903 bp	0.25	49.8