Hotspot

orf532-orf258

IRa · 177203-182508 · 5306 bp

Back to Oedogonium

Pi 0.46

MarkerSeek score 61.30

Primer available yes

Label orf532-orf258

Feature Metadata

label_name: orf532-orf258
feature_type: igs
region: IRa
length_bp: 5306
variable_sites: 1740
pi: 0.46
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.10
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf532-orf258_p1	AGCGAAAGACCTTTGTGTGT	ACGACAAACTGCGCTCTATT	45.0	45.0	58.0	57.9	1334 bp	0.25	49.1
orf532-orf258_p2	AGCGAAAGACCTTTGTGTGT	ATTACGACAAACTGCGCTCT	45.0	45.0	58.0	57.9	1337 bp	0.25	49.1
orf532-orf258_p3	GGTTGGCTGAGTATTACCCG	ACGACAAACTGCGCTCTATT	55.0	45.0	58.1	57.9	1124 bp	0.25	49.0
orf532-orf258_p4	GGTTGGCTGAGTATTACCCG	ATTACGACAAACTGCGCTCT	55.0	45.0	58.1	57.9	1127 bp	0.25	49.0
orf532-orf258_p5	AGCGAAAGACCTTTGTGTGT	TACTTGGCGCAACATTTCCT	45.0	45.0	58.0	58.1	1525 bp	0.25	48.9