Hotspot

orf458-rps12

LSC · 61950-62873 · 924 bp

Back to Oedogonium

Pi 0.136
MarkerSeek score 83.50
Primer available yes
Label orf458-rps12

Feature Metadata

label_name
orf458-rps12
feature_type
igs
region
LSC
length_bp
924
variable_sites
200
pi
0.136
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.94
markerseek_score
83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
orf458-rps12_p1 TCGATTTGAGTGCGCTGTTA TTTAGTTCGTGGTGGTCGTG 45.0 50.0 57.9 58.1 1261-1268 bp 0.5 59.8
orf458-rps12_p2 TCGATTTGAGTGCGCTGTTA CGTGGTGGTCGTGTAAAAGA 45.0 50.0 57.9 58.1 1254-1261 bp 0.5 59.7
orf458-rps12_p3 TCGATTTGAGTGCGCTGTTA TCGTGGTGGTCGTGTAAAAG 45.0 50.0 57.9 58.1 1255-1262 bp 0.5 59.7
orf458-rps12_p4 GGGTTCGAGGGCTGTTTTTA CGTGGTGGTCGTGTAAAAGA 50.0 50.0 58.1 58.1 1524-1531 bp 0.5 59.7
orf458-rps12_p5 GGGTTCGAGGGCTGTTTTTA TCGTGGTGGTCGTGTAAAAG 50.0 50.0 58.1 58.1 1525-1532 bp 0.5 59.7