Hotspot

petD

SSC · 137512-138800 · 1289 bp

Back to Oedocladium

Pi 0.398

MarkerSeek score 73.10

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: SSC
length_bp: 1289
variable_sites: 213
pi: 0.398
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.42
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TACGCGGAAGAGAAGAGTCT	TCCAGCTGCTATTGGTGAAC	50.0	50.0	57.9	57.9	1966 bp	0.5	58.2
petD_p2	GAGTTTCTGGCTTCGAACCA	TCCAGCTGCTATTGGTGAAC	50.0	50.0	58.1	57.9	1916 bp	0.5	58.1
petD_p3	GGGGTTCAATTCCTCTTGGG	TCCAGCTGCTATTGGTGAAC	55.0	50.0	58.1	57.9	1888 bp	0.5	57.9
petD_p4	TTATGGGGCACTTGCTCTTG	TCCAGCTGCTATTGGTGAAC	50.0	50.0	58.2	57.9	2302 bp	0.5	57.8
petD_p5	AGAGTTTCTGGCTTCGAACC	TCCAGCTGCTATTGGTGAAC	50.0	50.0	57.8	57.9	1917 bp	0.5	57.7