Hotspot

orf482

IRa · 196994-198442 · 1449 bp

Back to Oedocladium

Pi 0.335

MarkerSeek score 73.50

Primer available yes

Label orf482

Feature Metadata

label_name: orf482
feature_type: gene
region: IRa
length_bp: 1449
variable_sites: 52
pi: 0.335
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.11
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf482_p1	TGATATTGAAATCACTCTCCTGT	AGTCAGCTGGAATGTGGAAC	34.8	50.0	55.1	57.8	1562 bp	0.5	20.0
orf482_p2	ATTGAAATCACTCTCCTGTT	AGTCAGCTGGAATGTGGAAC	35.0	50.0	52.0	57.8	1558 bp	0.5	20.0
orf482_p3	TTGAAATCACTCTCCTGTTAG	AGTCAGCTGGAATGTGGAAC	38.1	50.0	52.9	57.8	1557 bp	0.5	20.0
orf482_p4	TGATATTGAAATCACTCTCCTGT	GTTGTCCCGGCCCTAATAAA	34.8	50.0	55.1	57.6	1511 bp	0.5	20.0
orf482_p5	TGATATTGAAATCACTCTCCTGTT	AGTCAGCTGGAATGTGGAAC	33.3	50.0	55.9	57.8	1562 bp	0.5	20.0