Hotspot

petB-trnG(gcc)

SSC · 160496-161523 · 1028 bp

Back to Oedocladium

Pi 0.353

MarkerSeek score 76.70

Primer available yes

Label petB-trnG(gcc)

Feature Metadata

label_name: petB-trnG(gcc)
feature_type: igs
region: SSC
length_bp: 1028
variable_sites: 187
pi: 0.353
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.51
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-trnG(gcc)_p1	ACGTTCTTCAAACCAATCGT	GCGGGTATAGTTCAGGGGTA	40.0	55.0	55.9	58.3	670-1133 bp	1.0	73.0
petB-trnG(gcc)_p2	AACGTTCTTCAAACCAATCGT	GCGGGTATAGTTCAGGGGTA	38.1	55.0	56.7	58.3	671-1134 bp	1.0	71.1
petB-trnG(gcc)_p3	AAACGTTCTTCAAACCAATCGT	GCGGGTATAGTTCAGGGGTA	36.4	55.0	57.4	58.3	672-1135 bp	1.0	68.7
petB-trnG(gcc)_p4	ACGTTCTTCAAACCAATCGT	CTTGCCAAGGAGGATGTCG	40.0	57.9	55.9	58.2	641-1104 bp	1.0	65.5
petB-trnG(gcc)_p5	ACGTTCTTCAAACCAATCGT	CGGGTATAGTTCAGGGGTAGA	40.0	52.4	55.9	57.8	669-1132 bp	1.0	65.4