Hotspot

psbB-trnN(guu)

SSC · 146893-147226 · 334 bp

Back to Oedocladium

Pi 0.346
MarkerSeek score 81.10
Primer available yes
Label psbB-trnN(guu)

Feature Metadata

label_name
psbB-trnN(guu)
feature_type
igs
region
SSC
length_bp
334
variable_sites
107
pi
0.346
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbB-trnN(guu)_p1 CACGATACCAAGGTAATCCCA CCGATTGGTCATAGGTTCAA 47.6 45.0 57.2 55.1 391-572 bp 1.0 54.5
psbB-trnN(guu)_p2 ACACGATACCAAGGTAATCCC CCGATTGGTCATAGGTTCAA 47.6 45.0 57.2 55.1 392-573 bp 1.0 54.5
psbB-trnN(guu)_p3 CACGATACCAAGGTAATCCCA CCGATTGGTCATAGGTTCAAA 47.6 42.9 57.2 56.0 391-572 bp 1.0 53.0
psbB-trnN(guu)_p4 ACACGATACCAAGGTAATCCC CCGATTGGTCATAGGTTCAAA 47.6 42.9 57.2 56.0 392-573 bp 1.0 53.0
psbB-trnN(guu)_p5 CACGATACCAAGGTAATCCCAT CCGATTGGTCATAGGTTCAA 45.5 45.0 57.6 55.1 391-572 bp 1.0 52.0