Hotspot

orf252-orf482

IRb · 101129-104883 · 3755 bp

Back to Oedocladium

Pi 0.17

MarkerSeek score 68.90

Primer available yes

Label orf252-orf482

Feature Metadata

label_name: orf252-orf482
feature_type: igs
region: IRb
length_bp: 3755
variable_sites: 174
pi: 0.17
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.27
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf252-orf482_p1	AAAGAACGAAGAGTCCTCGC	GAGATAGCAAGACGGTGTGG	50.0	55.0	57.9	58.1	1135 bp	0.5	58.9
orf252-orf482_p2	AAAGAACGAAGAGTCCTCGC	TAGCAAGACGGTGTGGATTG	50.0	50.0	57.9	57.9	1131 bp	0.5	58.7
orf252-orf482_p3	CAGCCAAGGACGTGTTATCA	GAGATAGCAAGACGGTGTGG	50.0	55.0	57.9	58.1	1362 bp	0.5	58.7
orf252-orf482_p4	TATCAACACTTGGGCTGACG	GAGATAGCAAGACGGTGTGG	50.0	55.0	57.9	58.1	1347 bp	0.5	58.7
orf252-orf482_p5	ACAGCCAAGGACGTGTTATC	GAGATAGCAAGACGGTGTGG	50.0	55.0	57.9	58.1	1363 bp	0.5	58.7