Hotspot

trnR(ucu)-psbA

LSC · 96895-99670 · 2776 bp

Back to Oedocladium

Pi 0.338

MarkerSeek score 68.50

Primer available yes

Label trnR(ucu)-psbA

Feature Metadata

label_name: trnR(ucu)-psbA
feature_type: igs
region: LSC
length_bp: 2776
variable_sites: 348
pi: 0.338
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.37
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR(ucu)-psbA_p1	CGATAAGTCGTGGGTTCGAA	AAGCATCCGAACCAACCAAT	50.0	45.0	57.7	58.1	1224-2917 bp	1.0	89.2
trnR(ucu)-psbA_p2	CGATAAGTCGTGGGTTCGAA	GAGCCCAAGTGCCTTTTACT	50.0	50.0	57.7	58.1	1163-2856 bp	1.0	89.2
trnR(ucu)-psbA_p3	CGATAAGTCGTGGGTTCGAA	TGCGATGAATGCGATGATGA	50.0	45.0	57.7	58.1	1279-2972 bp	1.0	88.8
trnR(ucu)-psbA_p4	CGATAAGTCGTGGGTTCGAA	CACAAAAACGAGCCCAAGTG	50.0	50.0	57.7	58.2	1172-2865 bp	1.0	88.6
trnR(ucu)-psbA_p5	CGATAAGTCGTGGGTTCGAA	CTGCGATGAATGCGATGATG	50.0	50.0	57.7	57.8	1280-2973 bp	1.0	88.0