Hotspot

psaJ-trnMf(cau)

LSC · 77689-77785 · 97 bp

Back to Oedocladium

Pi 0.159

MarkerSeek score 82.10

Primer available yes

Label psaJ-trnMf(cau)

Feature Metadata

label_name: psaJ-trnMf(cau)
feature_type: igs
region: LSC
length_bp: 97
variable_sites: 14
pi: 0.159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-trnMf(cau)_p1	GTGATCCAAGCAAATGCCAC	CGAGGTCGTAGGTTCGAATC	50.0	55.0	58.0	57.9	188-199 bp	1.0	88.7
psaJ-trnMf(cau)_p2	GTGATCCAAGCAAATGCCAC	CTTGTGGGGCTCATAATCCC	50.0	55.0	58.0	58.0	207-218 bp	1.0	88.6
psaJ-trnMf(cau)_p3	GTGATCCAAGCAAATGCCAC	AGGTCGTAGGTTCGAATCCT	50.0	50.0	58.0	57.9	186-197 bp	1.0	88.5
psaJ-trnMf(cau)_p4	AGTGATCCAAGCAAATGCCA	CTTGTGGGGCTCATAATCCC	45.0	55.0	58.1	58.0	208-219 bp	1.0	87.9
psaJ-trnMf(cau)_p5	GCCAGCTGTGATAGTGATCC	CTTGTGGGGCTCATAATCCC	55.0	55.0	58.1	58.0	220-231 bp	1.0	87.1