Hotspot

rpoBb-tufA

LSC · 55746-56562 · 817 bp

Back to Oedocladium

Pi 0.427

MarkerSeek score 83.50

Primer available yes

Label rpoBb-tufA

Feature Metadata

label_name: rpoBb-tufA
feature_type: igs
region: LSC
length_bp: 817
variable_sites: 328
pi: 0.427
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoBb-tufA_p1	ATATATCACCAGGCTGCACC	GGTAGCAACAGGACGTGTAG	50.0	55.0	57.1	58.0	1494 bp	0.5	53.0
rpoBb-tufA_p2	ATATATCACCAGGCTGCACC	CCCAACACCAGAACGAGAAA	50.0	50.0	57.1	58.0	1572 bp	0.5	52.8
rpoBb-tufA_p3	ATATATCACCAGGCTGCACC	TTTAGCACAACCCGGAACAA	50.0	45.0	57.1	57.9	1296 bp	0.5	52.6
rpoBb-tufA_p4	ATATATCACCAGGCTGCACC	TTTTAGCACAACCCGGAACA	50.0	45.0	57.1	57.9	1297 bp	0.5	52.6
rpoBb-tufA_p5	ATATATCACCAGGCTGCACC	ACCCGGAACAATTACACCAC	50.0	50.0	57.1	58.1	1287 bp	0.5	52.3