Hotspot

rpl14-psbD

LSC · 49030-50491 · 1462 bp

Back to Oedocladium

Pi 0.267

MarkerSeek score 66.40

Primer available yes

Label rpl14-psbD

Feature Metadata

label_name: rpl14-psbD
feature_type: igs
region: LSC
length_bp: 1462
variable_sites: 104
pi: 0.267
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.27
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-psbD_p1	CTTGGCTCCAGAAGTGGTTT	AGGAGCAAAGAACCAACCAG	50.0	50.0	58.0	58.0	2018 bp	0.5	59.8
rpl14-psbD_p2	CTTGGCTCCAGAAGTGGTTT	ACCAGCCTGACCTAAAGGAT	50.0	50.0	58.0	58.0	2003 bp	0.5	59.7
rpl14-psbD_p3	CTTGGCTCCAGAAGTGGTTT	ATCCACATCATCGAACCACG	50.0	50.0	58.0	58.1	1562 bp	0.5	59.4
rpl14-psbD_p4	GGGAAAGGTGCACCACAATA	AGGAGCAAAGAACCAACCAG	50.0	50.0	58.1	58.0	2736 bp	0.5	59.2
rpl14-psbD_p5	CTTGGCTCCAGAAGTGGTTT	AGAGCAACAAACGGCCATAA	50.0	45.0	58.0	58.1	1852 bp	0.5	59.1