Hotspot

rpl23-trnS(uga)

LSC · 44292-45092 · 801 bp

Back to Oedocladium

Pi 0.327
MarkerSeek score 75.70
Primer available yes
Label rpl23-trnS(uga)

Feature Metadata

label_name
rpl23-trnS(uga)
feature_type
igs
region
LSC
length_bp
801
variable_sites
145
pi
0.327
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.55
markerseek_score
75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl23-trnS(uga)_p1 GCCTAGCCTCCCGAAAATAC AATCCAGCAGCAACAACAGA 55.0 45.0 58.1 58.0 1787 bp 0.5 58.9
rpl23-trnS(uga)_p2 GCCTAGCCTCCCGAAAATAC TTACCACTTGACTATGCCGC 55.0 50.0 58.1 58.0 1472 bp 0.5 58.9
rpl23-trnS(uga)_p3 GCCTAGCCTCCCGAAAATAC AAGGAAAGGGAGGGATTCGA 55.0 50.0 58.1 58.0 1423 bp 0.5 58.8
rpl23-trnS(uga)_p4 GCCTAGCCTCCCGAAAATAC AGGAAAGGGAGGGATTCGAA 55.0 50.0 58.1 58.0 1422 bp 0.5 58.8
rpl23-trnS(uga)_p5 GCCTAGCCTCCCGAAAATAC GAAAGGGAGGGATTCGAACC 55.0 55.0 58.1 58.0 1420 bp 0.5 58.7