Hotspot

ccsA-psaC

IRa · 113444-114489 · 1046 bp

Back to Odontostemma

Pi 0.0957

MarkerSeek score 82.20

Primer available yes

Label ccsA-psaC

Feature Metadata

label_name: ccsA-psaC
feature_type: igs
region: IRa
length_bp: 1046
variable_sites: 177
pi: 0.0957
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-psaC_p1	GATGGTCCTGGGTTATGCAG	ATACCTTGGGACGGGTGTAA	55.0	50.0	58.0	58.0	1284-2425 bp	1.0	91.4
ccsA-psaC_p2	TGCAGCTCTTTTATGCGGAT	ATACCTTGGGACGGGTGTAA	45.0	50.0	58.0	58.0	1269-2410 bp	1.0	91.3
ccsA-psaC_p3	GAGCAGTATGGGCCAATGAA	ATACCTTGGGACGGGTGTAA	50.0	50.0	57.9	58.0	988-2129 bp	1.0	91.2
ccsA-psaC_p4	ATGCAGCTCTTTTATGCGGA	ATACCTTGGGACGGGTGTAA	45.0	50.0	58.0	58.0	1270-2411 bp	0.833333	83.9
ccsA-psaC_p5	GATGGTCCTGGGTTATGCAG	TCGGAATCTATGGACCGGAA	55.0	50.0	58.0	57.9	2295-2320 bp	0.333333	52.6