Hotspot

trnT-UGU-trnL-UAA

LSC · 43389-44028 · 640 bp

Back to Odontostemma

Pi 0.043

MarkerSeek score 79.80

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 640
variable_sites: 55
pi: 0.043
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	AGCCAATTAAGTCCGTAGCG	55.0	50.0	57.9	58.1	484-720 bp	1.0	89.1
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	AGCCAATTAAGTCCGTAGCG	50.0	50.0	57.8	58.1	516-752 bp	1.0	87.9
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	472-708 bp	1.0	87.7
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	GCCAATTAAGTCCGTAGCGT	55.0	50.0	57.9	58.4	483-719 bp	1.0	86.8
trnT-UGU-trnL-UAA_p5	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	504-740 bp	1.0	86.6