Hotspot

psaA-ycf3

LSC · 39224-39946 · 723 bp

Back to Odontostemma

Pi 0.041

MarkerSeek score 80.30

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 723
variable_sites: 67
pi: 0.041
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCATCAGCATGTAGGTTCCA	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.0	58.0	973-1234 bp	1.0	88.7
psaA-ycf3_p2	GGCACTAGGTCCAATGTGAG	ATCAAGCCGCTGAGTATTGG	55.0	50.0	58.0	58.0	1151-1412 bp	1.0	87.9
psaA-ycf3_p3	ATCGCTTAGCCATGCTTCAA	ATCAAGCCGCTGAGTATTGG	45.0	50.0	58.0	58.0	1127-1388 bp	1.0	87.9
psaA-ycf3_p4	GCCTACGATTGGCCAAACTA	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.0	58.0	1175-1436 bp	1.0	87.7
psaA-ycf3_p5	TGAGTAGGATCGCTTAGCCA	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	1135-1396 bp	1.0	87.7