Hotspot

atpF-atpH

LSC · 12112-12584 · 473 bp

Back to Odontostemma

Pi 0.0505

MarkerSeek score 86.90

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 473
variable_sites: 47
pi: 0.0505
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 86.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CCAGTGACCCAAGAAAACGA	CCGAGGGAAAAATACGAGGT	50.0	50.0	58.0	57.3	522-633 bp	1.0	84.4
atpF-atpH_p2	CCAGTGACCCAAGAAAACGA	GCCGAGGGAAAAATACGAGG	50.0	55.0	58.0	58.7	523-634 bp	1.0	84.3
atpF-atpH_p3	CCAGTGACCCAAGAAAACGA	CGAGGCCGAGGGAAAAATAC	50.0	55.0	58.0	58.7	527-638 bp	1.0	84.2
atpF-atpH_p4	CCAGTGACCCAAGAAAACGA	GAGGCCGAGGGAAAAATACG	50.0	55.0	58.0	58.7	526-637 bp	1.0	84.2
atpF-atpH_p5	CCAGTGACCCAAGAAAACGA	GGCCGAGGGAAAAATACGAG	50.0	55.0	58.0	58.7	524-635 bp	1.0	84.2