Hotspot

trnS-GCU-trnG-UCC

LSC · 7703-8197 · 495 bp

Back to Odontostemma

Pi 0.0589

MarkerSeek score 82.50

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 495
variable_sites: 60
pi: 0.0589
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	618-882 bp	1.0	58.5
trnS-GCU-trnG-UCC_p2	ACGGAAAGAGAGGGATTCGA	AACGAATCACACTTTTACCACT	50.0	36.4	58.2	56.2	619-883 bp	1.0	56.5
trnS-GCU-trnG-UCC_p3	CTTTCGTCCACTCAGCCATC	ACGAATCACACTTTTACCACT	55.0	38.1	58.6	55.5	554-818 bp	1.0	55.3
trnS-GCU-trnG-UCC_p4	ACGGAAAGAGAGGGATTCGA	TACCACTAAACTATACCCGC	50.0	45.0	58.2	54.0	604-868 bp	1.0	54.8
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCAC	50.0	40.0	58.2	53.9	618-882 bp	1.0	53.8