Hotspot

rps16-trnQ-UUG

LSC · 5799-6404 · 606 bp

Back to Odontostemma

Pi 0.0585

MarkerSeek score 81.80

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 606
variable_sites: 70
pi: 0.0585
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	ATAAAACGGATTCGCGTGGA	CGAAAACTGACAGCAGCTTG	45.0	50.0	58.0	58.0	1046-1362 bp	1.0	91.4
rps16-trnQ-UUG_p2	ATAAAACGGATTCGCGTGGA	TCGAAAACTGACAGCAGCTT	45.0	45.0	58.0	58.0	1047-1363 bp	1.0	91.3
rps16-trnQ-UUG_p3	CCCAATAAAACGGATTCGCG	CGAAAACTGACAGCAGCTTG	50.0	50.0	57.9	58.0	1050-1366 bp	1.0	90.7
rps16-trnQ-UUG_p4	CCACAACGGATCATGTCCTT	CGAAAACTGACAGCAGCTTG	50.0	50.0	57.9	58.0	967-1283 bp	1.0	90.4
rps16-trnQ-UUG_p5	ATAAAACGGATTCGCGTGGA	GCTATTCGGAGGTTCGAGTC	45.0	55.0	58.0	57.9	788 bp	0.333333	53.2