Hotspot

trnK-UUU-rps16

LSC · 4071-4725 · 655 bp

Back to Odontostemma

Pi 0.05

MarkerSeek score 82.70

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 655
variable_sites: 65
pi: 0.05
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TTCCCGTTTCAGGCACTAAG	AAAAAGGCGCTCAACCTACA	50.0	45.0	57.8	58.0	967-1158 bp	1.0	88.8
trnK-UUU-rps16_p2	TTCCCGTTTCAGGCACTAAG	CGGTCACGAAGAGAAGGAAG	50.0	55.0	57.8	58.0	1067-1258 bp	1.0	88.6
trnK-UUU-rps16_p3	TTCCCGTTTCAGGCACTAAG	CGAGCCGTACGAAGAGAAAA	50.0	50.0	57.8	58.0	1159-1350 bp	1.0	88.3
trnK-UUU-rps16_p4	TTCCCGTTTCAGGCACTAAG	TTTTCTCGAGCCGTACGAAG	50.0	50.0	57.8	58.0	1165-1356 bp	1.0	88.3
trnK-UUU-rps16_p5	ACTCGTGGCCATCTCCATAT	CGGTCACGAAGAGAAGGAAG	50.0	55.0	58.3	58.0	1159-1350 bp	1.0	87.6