Hotspot

rpoC1

LSC · 19833-22640 · 2808 bp

Back to Odontochilus

Pi 0.00427

MarkerSeek score 58.50

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2808
variable_sites: 12
pi: 0.00427
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	CCAGTGAAATTGGGGGAGAC	TTGCTCGTTCGAGAACTACG	55.0	50.0	58.2	58.0	2995 bp	1.0	78.7
rpoC1_p2	CCAGTGAAATTGGGGGAGAC	CTTTGGCCCTGGAACTGAAT	55.0	50.0	58.2	58.1	2973 bp	1.0	78.5
rpoC1_p3	CCAGTGAAATTGGGGGAGAC	TTTGGCCCTGGAACTGAATC	55.0	50.0	58.2	58.1	2972 bp	1.0	78.4
rpoC1_p4	GAGACCCAAATTCCCACGAG	TTGCTCGTTCGAGAACTACG	55.0	50.0	58.3	58.0	2980 bp	1.0	77.9
rpoC1_p5	CCAGTGAAATTGGGGGAGAC	GGCCCTGGAACTGAATCATT	55.0	50.0	58.2	57.9	2969 bp	1.0	77.8