Hotspot

rpl20-rps12

LSC · 67473-68267 · 795 bp

Back to Odontochilus

Pi 0.00377

MarkerSeek score 60.90

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 795
variable_sites: 3
pi: 0.00377
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	GTCAGTCTTGAATGAGCCCC	GAAATCTCCCGCTCTTCGAG	55.0	55.0	58.3	58.2	948-949 bp	1.0	77.0
rpl20-rps12_p2	TCAGTCTTGAATGAGCCCCT	GAAATCTCCCGCTCTTCGAG	50.0	55.0	58.3	58.2	947-948 bp	1.0	76.3
rpl20-rps12_p3	GTCAGTCTTGAATGAGCCCC	TAGAAACGCAAGACAGCCAA	55.0	45.0	58.3	57.8	981-982 bp	1.0	76.2
rpl20-rps12_p4	GTCAGTCTTGAATGAGCCCC	TTAGAAACGCAAGACAGCCA	55.0	45.0	58.3	57.8	982-983 bp	1.0	76.2
rpl20-rps12_p5	TCAGTCTTGAATGAGCCCCT	TAGAAACGCAAGACAGCCAA	50.0	45.0	58.3	57.8	980-981 bp	1.0	75.6