Hotspot

trnW-CCA-trnP-UGG

LSC · 65008-65167 · 160 bp

Back to Odontochilus

Pi 0.0187
MarkerSeek score 63.30
Primer available yes
Label trnW-CCA-trnP-UGG

Feature Metadata

label_name
trnW-CCA-trnP-UGG
feature_type
igs
region
LSC
length_bp
160
variable_sites
3
pi
0.0187
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-CCA-trnP-UGG_p1 TGGAATGGAATCACGCTCTG GGGTACAAAATGTCACGGGT 50.0 50.0 58.0 58.1 284 bp 1.0 79.8
trnW-CCA-trnP-UGG_p2 TGGAATGGAATCACGCTCTG TGGGTACAAAATGTCACGGG 50.0 50.0 58.0 58.1 285 bp 1.0 79.8
trnW-CCA-trnP-UGG_p3 GGAATGGAATCACGCTCTGT GGGTACAAAATGTCACGGGT 50.0 50.0 58.0 58.1 283 bp 1.0 79.8
trnW-CCA-trnP-UGG_p4 GGAATGGAATCACGCTCTGT TGGGTACAAAATGTCACGGG 50.0 50.0 58.0 58.1 284 bp 1.0 79.8
trnW-CCA-trnP-UGG_p5 TGGAATGGAATCACGCTCTG GTCACGGGTTCAAATCCTGT 50.0 50.0 58.0 58.1 273 bp 1.0 79.8