Hotspot

psaA-ycf3

LSC · 40661-41380 · 720 bp

Back to Odontochilus

Pi 0.00556

MarkerSeek score 61.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 720
variable_sites: 4
pi: 0.00556
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	CTGAGCCGTATGAGGTAGGA	50.0	55.0	57.9	58.0	908 bp	1.0	79.5
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	GAATCGATTGCTGAGCCGTA	50.0	50.0	57.9	58.1	918 bp	1.0	78.4
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	ATCGATTGCTGAGCCGTATG	50.0	50.0	57.9	58.2	916 bp	1.0	77.9
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	GGGAGAACAGGCCATTCTAC	50.0	55.0	57.9	57.7	837 bp	1.0	77.0
psaA-ycf3_p5	TGTTCTTGAGAAATGGCCGG	CTGAGCCGTATGAGGTAGGA	50.0	55.0	58.5	58.0	934 bp	1.0	76.5