Hotspot

atpF-atpH

LSC · 12379-12761 · 383 bp

Back to Odontochilus

Pi 0.0131

MarkerSeek score 61.70

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 383
variable_sites: 5
pi: 0.0131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CCACGGAAACAAAAGAATCGG	ATTTATGGACTGGTGGTGGC	47.6	50.0	58.1	57.9	471-521 bp	1.0	71.1
atpF-atpH_p2	CCCACGGAAACAAAAGAATCG	ATTTATGGACTGGTGGTGGC	47.6	50.0	58.1	57.9	472-522 bp	1.0	71.1
atpF-atpH_p3	CCACGGAAACAAAAGAATCGG	GGTGGTGGCATTAGCTCTTT	47.6	50.0	58.1	58.2	460-510 bp	1.0	71.0
atpF-atpH_p4	CCCACGGAAACAAAAGAATCG	GGTGGTGGCATTAGCTCTTT	47.6	50.0	58.1	58.2	461-511 bp	1.0	71.0
atpF-atpH_p5	GCCCCACGGAAACAAAAGAA	ATTTATGGACTGGTGGTGGC	50.0	50.0	59.3	57.9	474-524 bp	1.0	69.6