Hotspot

trnK-UUU-rps16

LSC · 4297-4914 · 618 bp

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Pi 0.013
MarkerSeek score 54.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
618
variable_sites
8
pi
0.013
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT TCTTGAGCCGTATGAGGAGA 50.0 50.0 58.2 57.6 991 bp 1.0 76.1
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT TCTTGAGCCGTATGAGGAGA 50.0 50.0 58.2 57.6 990 bp 1.0 76.1
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT AGACAACGAATTCCGGAACA 50.0 45.0 58.2 57.5 1297-1313 bp 1.0 75.2
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT AGACAACGAATTCCGGAACA 50.0 45.0 58.2 57.5 1296-1312 bp 1.0 75.2
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT AACGAATTCCGGAACAAGGA 50.0 45.0 58.2 57.4 1292-1308 bp 1.0 75.0