Hotspot

ycf2

LSC · 90041-97011 · 6971 bp

Pi 0.00395

MarkerSeek score 54.90

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: LSC
length_bp: 6971
variable_sites: 75
pi: 0.00395
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	CGGAACAAAGGGGGATACAG	AGATTCGTGGATCTCTCGGT	55.0	50.0	58.0	57.9	736-737 bp	1.0	87.1
ycf2_p2	CGGAACAAAGGGGGATACAG	AACCTCGGACCAATCAATCG	55.0	50.0	58.0	58.0	887-888 bp	1.0	86.3
ycf2_p3	CGGAACAAAGGGGGATACAG	TCGGCCCTATGAATGGAGAT	55.0	50.0	58.0	58.0	1030-1031 bp	1.0	85.6
ycf2_p4	ATCCGGGATGGGATATGGTT	AACCTCGGACCAATCAATCG	50.0	50.0	57.9	58.0	997-998 bp	1.0	85.3
ycf2_p5	ATCCGGGATGGGATATGGTT	TCGGCCCTATGAATGGAGAT	50.0	50.0	57.9	58.0	1140-1141 bp	1.0	84.8