Hotspot

ycf1

SSC · 112451-118018 · 5568 bp

Back to Ocotea

Pi 0.00438
MarkerSeek score 39.90
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5568
variable_sites
73
pi
0.00438
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
39.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TGGTGGATGACTCAATCACT TGAATCAAATCTTCTTTGTTCGGA 45.0 33.3 56.1 57.1 251 bp 1.0 43.2
ycf1_p2 TGGTGGATGACTCAATCACTTC TGAATCAAATCTTCTTTGTTCGGA 45.5 33.3 58.1 57.1 251 bp 1.0 43.2
ycf1_p3 TGGTGGATGACTCAATCACT CTGAATCAAATCTTCTTTGTTCGGA 45.0 36.0 56.1 58.1 252 bp 1.0 43.2
ycf1_p4 TGGTGGATGACTCAATCACT TCTGAATCAAATCTTCTTTGTTCGG 45.0 36.0 56.1 58.1 253 bp 1.0 43.2
ycf1_p5 TGGTGGATGACTCAATCACTT TGAATCAAATCTTCTTTGTTCGGA 42.9 33.3 56.9 57.1 251 bp 1.0 43.2