Hotspot

trnL-UAG-rpl32

SSC · 127607-129006 · 1400 bp

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Pi 0.0101
MarkerSeek score 59.40
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
1400
variable_sites
39
pi
0.0101
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 TCCTAAGAGCAGCGTGTCTA TTTTTGTGCGACAAACAAGT 50.0 35.0 57.9 55.2 1451-1477 bp 1.0 61.0
trnL-UAG-rpl32_p2 AGCAGCGTGTCTACCAATTT TTTTTGTGCGACAAACAAGT 45.0 35.0 57.8 55.2 1444-1470 bp 1.0 60.6
trnL-UAG-rpl32_p3 CCTAAGAGCAGCGTGTCTAC TTTTTGTGCGACAAACAAGT 55.0 35.0 57.8 55.2 1450-1476 bp 1.0 60.4
trnL-UAG-rpl32_p4 CTAAGAGCAGCGTGTCTACC TTTTTGTGCGACAAACAAGT 55.0 35.0 57.8 55.2 1449-1475 bp 1.0 60.4
trnL-UAG-rpl32_p5 GAGCAGCGTGTCTACCAATT TTTTTGTGCGACAAACAAGT 50.0 35.0 58.3 55.2 1445-1471 bp 1.0 59.8