Hotspot

ccsA-trnL-UAG

SSC · 127463-127526 · 64 bp

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Pi 0.0982
MarkerSeek score 62.20
Primer available yes
Label ccsA-trnL-UAG

Feature Metadata

label_name
ccsA-trnL-UAG
feature_type
igs
region
SSC
length_bp
64
variable_sites
12
pi
0.0982
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-trnL-UAG_p1 TGTTCTAAAGTGGCAAATATCA TAGACACGCTGCTCTTAGGA 31.8 50.0 53.7 57.9 148-154 bp 1.0 46.4
ccsA-trnL-UAG_p2 TGTTCTAAAGTGGCAAATATCA GGTAGACACGCTGCTCTTAG 31.8 55.0 53.7 57.8 150-156 bp 1.0 46.4
ccsA-trnL-UAG_p3 TGTTCTAAAGTGGCAAATATCA GTAGACACGCTGCTCTTAGG 31.8 55.0 53.7 57.8 149-155 bp 1.0 46.4
ccsA-trnL-UAG_p4 TGTTCTAAAGTGGCAAATATCA AAATTGGTAGACACGCTGCT 31.8 45.0 53.7 57.8 155-161 bp 1.0 46.2
ccsA-trnL-UAG_p5 TGTTCTAAAGTGGCAAATATCA TTATTTCGATGAAGCCGCCA 31.8 45.0 53.7 58.0 180-186 bp 1.0 45.3