Hotspot

ycf1-rps15

SSC · 118019-118339 · 321 bp

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Pi 0.0121
MarkerSeek score 52.90
Primer available yes
Label ycf1-rps15

Feature Metadata

label_name
ycf1-rps15
feature_type
igs
region
SSC
length_bp
321
variable_sites
10
pi
0.0121
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-rps15_p1 AATGGCAGCCGTTTCAGTAT TGAAATTCAACAGACCCTTTGT 45.0 36.4 57.9 56.4 441-460 bp 1.0 54.2
ycf1-rps15_p2 ATAATGGCAGCCGTTTCAGT TGAAATTCAACAGACCCTTTGT 45.0 36.4 57.9 56.4 443-462 bp 1.0 54.2
ycf1-rps15_p3 AATGGCAGCCGTTTCAGTAT ACTTGAAATTCAACAGACCCT 45.0 38.1 57.9 55.3 444-463 bp 1.0 53.7
ycf1-rps15_p4 ATAATGGCAGCCGTTTCAGT ACTTGAAATTCAACAGACCCT 45.0 38.1 57.9 55.3 446-465 bp 1.0 53.7
ycf1-rps15_p5 CCAATAATGGCAGCCGTTTC TGAAATTCAACAGACCCTTTGT 50.0 36.4 57.8 56.4 446-465 bp 1.0 53.5