Hotspot

ndhF

SSC · 109104-111335 · 2232 bp

Pi 0.00608

MarkerSeek score 28.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 31
pi: 0.00608
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AAGGCGGATCCGAACAAAAT	AGAAAACGCTTTTAACGCCG	45.0	45.0	58.2	58.0	2966-2969 bp	1.0	79.8
ndhF_p2	GAAGAAAAGGCGGATCCGAA	AGAAAACGCTTTTAACGCCG	50.0	45.0	58.3	58.0	2972-2975 bp	1.0	79.0
ndhF_p3	AAAAGGCGGATCCGAACAAA	AGAAAACGCTTTTAACGCCG	45.0	45.0	58.4	58.0	2968-2971 bp	1.0	78.0
ndhF_p4	AAAGGCGGATCCGAACAAAA	AGAAAACGCTTTTAACGCCG	45.0	45.0	58.4	58.0	2967-2970 bp	1.0	78.0
ndhF_p5	AAGAAAAGGCGGATCCGAAC	AGAAAACGCTTTTAACGCCG	50.0	45.0	58.5	58.0	2971-2974 bp	1.0	76.7