Hotspot

psaC-ndhE

SSC · 115944-116203 · 260 bp

Back to Ocimum

Pi 0.00989
MarkerSeek score 29.60
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
260
variable_sites
6
pi
0.00989
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
29.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 ATACGCTTGGGCCAGAAAAA TGAAGCAGCTATTGGATCGG 45.0 50.0 58.1 57.8 697-702 bp 1.0 79.7
psaC-ndhE_p2 CTAATACATCGGTGGGGCAC TGAAGCAGCTATTGGATCGG 55.0 50.0 58.1 57.8 425-430 bp 1.0 79.3
psaC-ndhE_p3 ACCTCTTACAACCAACGCAG TGAAGCAGCTATTGGATCGG 50.0 50.0 58.1 57.8 506-511 bp 1.0 79.0
psaC-ndhE_p4 GAGAATCTCGAGTAACCGGC TGAAGCAGCTATTGGATCGG 55.0 50.0 57.9 57.8 1043-1048 bp 1.0 78.8
psaC-ndhE_p5 CCTTGGGGCAGAAGCTATTT TGAAGCAGCTATTGGATCGG 50.0 50.0 57.9 57.8 478-483 bp 1.0 78.8