Hotspot

trnR-ACG-trnN-GUU

IRb · 107096-107666 · 571 bp

Back to Ocimum

Pi 0.00525
MarkerSeek score 44.30
Primer available yes
Label trnR-ACG-trnN-GUU

Feature Metadata

label_name
trnR-ACG-trnN-GUU
feature_type
igs
region
IRb
length_bp
571
variable_sites
7
pi
0.00525
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-trnN-GUU_p1 CAGAGGATTAGAGCACGTGG CCGTTGTTCCTCAGTAGCTC 55.0 55.0 57.8 58.0 708-713 bp 1.0 79.6
trnR-ACG-trnN-GUU_p2 GAACAGATCCAGTGGAGACG CCGTTGTTCCTCAGTAGCTC 55.0 55.0 57.7 58.0 745-750 bp 1.0 79.0
trnR-ACG-trnN-GUU_p3 CAGAGGATTAGAGCACGTGG TGGTCGTAGGTTCGAATCCT 55.0 50.0 57.8 58.2 659-664 bp 1.0 78.5
trnR-ACG-trnN-GUU_p4 GAACAGATCCAGTGGAGACG TGGTCGTAGGTTCGAATCCT 55.0 50.0 57.7 58.2 696-701 bp 1.0 77.8
trnR-ACG-trnN-GUU_p5 CAGAGGATTAGAGCACGTGG CGATTGGTCGTAGGTTCGAA 55.0 50.0 57.8 57.7 663-668 bp 1.0 77.6