Hotspot

pafII-cemA

LSC · 60413-61060 · 648 bp

Back to Ocimum

Pi 0.00898
MarkerSeek score 47.80
Primer available yes
Label pafII-cemA

Feature Metadata

label_name
pafII-cemA
feature_type
igs
region
LSC
length_bp
648
variable_sites
14
pi
0.00898
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
pafII-cemA_p1 TGCGCGTACCAATTGAAGTA GAAATCCACCAGGGCAGAAA 45.0 50.0 57.9 58.1 691-745 bp 1.0 84.3
pafII-cemA_p2 CTTGCGCGTACCAATTGAAG GAAATCCACCAGGGCAGAAA 50.0 50.0 58.1 58.1 693-747 bp 1.0 84.0
pafII-cemA_p3 GAACAAAAAGCCGCGGAATT GAAATCCACCAGGGCAGAAA 45.0 50.0 58.2 58.1 722-776 bp 1.0 83.1
pafII-cemA_p4 TTTCTTGCGCGTACCAATTG GAAATCCACCAGGGCAGAAA 45.0 50.0 57.7 58.1 696-750 bp 1.0 82.3
pafII-cemA_p5 TGCGCGTACCAATTGAAGTA TCCACCAGGGCAGAAATACT 45.0 50.0 57.9 58.3 687-741 bp 1.0 82.2