Hotspot

rps16

LSC · 5299-6254 · 956 bp

Pi 0.00837

MarkerSeek score 50.70

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 956
variable_sites: 8
pi: 0.00837
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TCGGGTTTATTTTATACAATCAA	GCCGAGCCAATGACTATTCA	26.1	50.0	52.2	58.0	1056-1536 bp	1.0	40.5
rps16_p2	TCGGGTTTATTTTATACAATCAA	TACTATATACCGGCCGAGCC	26.1	55.0	52.2	58.2	1068-1548 bp	1.0	40.5
rps16_p3	TCGGGTTTATTTTATACAATCAAT	GCCGAGCCAATGACTATTCA	25.0	50.0	52.8	58.0	1056-1536 bp	1.0	40.5
rps16_p4	TCGGGTTTATTTTATACAATCAAT	TACTATATACCGGCCGAGCC	25.0	55.0	52.8	58.2	1068-1548 bp	1.0	40.5
rps16_p5	TCGGGTTTATTTTATACAATCAA	CGAGCCAATGACTATTCACGA	26.1	47.6	52.2	58.2	1054-1534 bp	1.0	40.5