Hotspot

rpl22

LSC · 87188-87619 · 432 bp

Pi 0.0162

MarkerSeek score 64.10

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 432
variable_sites: 7
pi: 0.0162
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	CCAAGTCTGAAACCAAGTGGA	TGGATTTTGATTTGAGGTGAACC	47.6	39.1	58.1	57.5	570-571 bp	1.0	44.7
rpl22_p2	ACCAAGTCTGAAACCAAGTGG	TGGATTTTGATTTGAGGTGAACC	47.6	39.1	58.3	57.5	571-572 bp	1.0	42.5
rpl22_p3	CCAAGTCTGAAACCAAGTGG	TGGATTTTGATTTGAGGTGAACC	50.0	39.1	56.5	57.5	570-571 bp	1.0	41.6
rpl22_p4	CCAAGTCTGAAACCAAGTGGA	GGATTTTGATTTGAGGTGAACC	47.6	40.9	58.1	55.9	569-570 bp	1.0	41.0
rpl22_p5	CCAAGTCTGAAACCAAGTGGA	TTGGATTTTGATTTGAGGTGAACC	47.6	37.5	58.1	58.1	571-572 bp	1.0	41.0