Hotspot

ccsA

SSC · 116946-117917 · 972 bp

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Pi 0.00514
MarkerSeek score 61.70
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
SSC
length_bp
972
variable_sites
5
pi
0.00514
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 TGAAGGACTCTCCCGTCTTT CGCCAGACCCTTTACTTGAA 50.0 50.0 58.0 57.8 1025 bp 1.0 78.9
ccsA_p2 TGAAGGACTCTCCCGTCTTT TTCGCCAGACCCTTTACTTG 50.0 50.0 58.0 57.8 1027 bp 1.0 78.9
ccsA_p3 TTGAAGGACTCTCCCGTCTT CGCCAGACCCTTTACTTGAA 50.0 50.0 58.0 57.8 1026 bp 1.0 78.9
ccsA_p4 TTGAAGGACTCTCCCGTCTT TTCGCCAGACCCTTTACTTG 50.0 50.0 58.0 57.8 1028 bp 1.0 78.9
ccsA_p5 TGAAGGACTCTCCCGTCTTT GGGTTATATTCGCCAGACCC 50.0 55.0 58.0 57.8 1035 bp 1.0 78.9