Hotspot

ycf4-cemA

LSC · 64070-64638 · 569 bp

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Pi 0.00879
MarkerSeek score 53.20
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
569
variable_sites
5
pi
0.00879
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 TTGGCCTATTTCTTGCGTGT CACCAGGGCAAAAGGGTTAT 45.0 50.0 58.1 58.1 670 bp 1.0 79.3
ycf4-cemA_p2 TTGGCCTATTTCTTGCGTGT AGATACACCAGGGCAAAAGG 45.0 50.0 58.1 57.5 675 bp 1.0 75.7
ycf4-cemA_p3 TTGGCCTATTTCTTGCGTGT GATACACCAGGGCAAAAGGG 45.0 55.0 58.1 58.5 674 bp 1.0 75.6
ycf4-cemA_p4 TGGCCTATTTCTTGCGTGTA CACCAGGGCAAAAGGGTTAT 45.0 50.0 57.2 58.1 669 bp 1.0 73.9
ycf4-cemA_p5 CGGCTGAATTGGCCTATTTC CACCAGGGCAAAAGGGTTAT 50.0 50.0 57.2 58.1 678 bp 1.0 73.8