Hotspot

rbcL-accD

LSC · 59850-60708 · 859 bp

Back to Ochna

Pi 0.0035
MarkerSeek score 60.90
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
859
variable_sites
3
pi
0.0035
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 GAGTCCTGAACTAGCTGCTG AGCATCGAATTGAACCACCA 55.0 45.0 57.7 57.8 964 bp 1.0 76.4
rbcL-accD_p2 GCTGCTGCTTGTGAAGTATG AGCATCGAATTGAACCACCA 50.0 45.0 57.5 57.8 951 bp 1.0 74.4
rbcL-accD_p3 TGAAATTATACGCGAGGCCA AGCATCGAATTGAACCACCA 45.0 45.0 57.4 57.8 991 bp 1.0 73.9
rbcL-accD_p4 CTTGCTCGTGAGGGTAATGA AGCATCGAATTGAACCACCA 50.0 45.0 57.3 57.8 1008 bp 1.0 73.3
rbcL-accD_p5 AAATTATACGCGAGGCCAGC AGCATCGAATTGAACCACCA 50.0 45.0 58.8 57.8 989 bp 1.0 72.5