Hotspot

trnT-GGU-psbD

LSC · 34187-35715 · 1529 bp

Back to Ochna

Pi 0.00393
MarkerSeek score 61.00
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1529
variable_sites
6
pi
0.00393
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1678-1679 bp 1.0 77.9
trnT-GGU-psbD_p2 GTGGTAGAGTAACGCCATGG GACCGGACCAACCTACAAAA 55.0 50.0 58.1 57.7 1692-1693 bp 1.0 77.7
trnT-GGU-psbD_p3 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1681-1682 bp 1.0 77.7
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA GACCGGACCAACCTACAAAA 50.0 50.0 58.1 57.7 1695-1696 bp 1.0 77.6
trnT-GGU-psbD_p5 GTGGTAGAGTAACGCCATGG TAGACCGGACCAACCTACAA 55.0 50.0 58.1 57.7 1694-1695 bp 1.0 77.4