Hotspot

trnE-UUC-trnT-GGU

LSC · 33153-34114 · 962 bp

Back to Ochna

Pi 0.00422
MarkerSeek score 62.70
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
962
variable_sites
4
pi
0.00422
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 AGAGATGTCCTGAACCGCTA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 1044-1049 bp 1.0 80.2
trnE-UUC-trnT-GGU_p2 AGATGTCCTGAACCGCTAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 1042-1047 bp 1.0 80.2
trnE-UUC-trnT-GGU_p3 AGAGATGTCCTGAACCGCTA CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 1023-1028 bp 1.0 79.8
trnE-UUC-trnT-GGU_p4 AGATGTCCTGAACCGCTAGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 1021-1026 bp 1.0 79.8
trnE-UUC-trnT-GGU_p5 GATGTCCTGAACCGCTAGAC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 1041-1046 bp 1.0 79.7