Hotspot

psbM-trnD-GUC

LSC · 31229-32449 · 1221 bp

Back to Ochna

Pi 0.00656
MarkerSeek score 62.70
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1221
variable_sites
8
pi
0.00656
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGAATGAAGAGTGCAGTAGC TTCAATTGGTCAGAGCACCG 45.0 50.0 55.2 58.5 1330-1332 bp 1.0 54.2
psbM-trnD-GUC_p2 ACTAGAATGAAGAGTGCAGTAGC TTCAATTGGTCAGAGCACCG 43.5 50.0 58.0 58.5 1333-1335 bp 1.0 52.7
psbM-trnD-GUC_p3 AGGAACTAGAATGAAGAGTGCAG TTCAATTGGTCAGAGCACCG 43.5 50.0 57.9 58.5 1337-1339 bp 1.0 52.1
psbM-trnD-GUC_p4 AGGAACTAGAATGAAGAGTGCA TTCAATTGGTCAGAGCACCG 40.9 50.0 56.8 58.5 1337-1339 bp 1.0 51.4
psbM-trnD-GUC_p5 GGAACTAGAATGAAGAGTGCAGT TTCAATTGGTCAGAGCACCG 43.5 50.0 58.2 58.5 1336-1338 bp 1.0 51.4