Hotspot

rpoB-trnC-GCA

LSC · 28169-29371 · 1203 bp

Back to Ochna

Pi 0.00583
MarkerSeek score 53.30
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1203
variable_sites
7
pi
0.00583
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 CCCTCATTTCCATTCCCGAG AAAAGGATTTGCAGTCCCCC 55.0 50.0 58.0 58.4 1269-1270 bp 1.0 77.5
rpoB-trnC-GCA_p2 CCCTCATTTCCATTCCCGAG CGGGGAAAAAGGATTTGCAG 55.0 50.0 58.0 57.6 1275-1276 bp 1.0 77.4
rpoB-trnC-GCA_p3 CTCATTTCCATTCCCGAGCA AAAAGGATTTGCAGTCCCCC 50.0 50.0 58.0 58.4 1267-1268 bp 1.0 77.4
rpoB-trnC-GCA_p4 CTCATTTCCATTCCCGAGCA CGGGGAAAAAGGATTTGCAG 50.0 50.0 58.0 57.6 1273-1274 bp 1.0 77.3
rpoB-trnC-GCA_p5 CCCTCATTTCCATTCCCGAG TTTGAACCGGGGAAAAAGGA 55.0 45.0 58.0 57.5 1282-1283 bp 1.0 76.7