Hotspot

atpF-atpH

LSC · 14109-14603 · 495 bp

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Pi 0.0121
MarkerSeek score 63.50
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
495
variable_sites
6
pi
0.0121
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 GCCCCAAGGAAAGGAAAGAA AGCGGAGGGAAAAATACGAG 50.0 50.0 58.0 57.7 647-656 bp 1.0 78.2
atpF-atpH_p2 ATTAAACCCGAAACTCCCGG AGCGGAGGGAAAAATACGAG 50.0 50.0 57.9 57.7 679-688 bp 1.0 77.3
atpF-atpH_p3 GCCCCAAGGAAAGGAAAGAA CGGAGGGAAAAATACGAGGT 50.0 50.0 58.0 57.3 645-654 bp 1.0 75.2
atpF-atpH_p4 GCCCCAAGGAAAGGAAAGAA GCGGAGGGAAAAATACGAGG 50.0 55.0 58.0 58.7 646-655 bp 1.0 75.0
atpF-atpH_p5 GCCCCAAGGAAAGGAAAGAA AAGCGGAGGGAAAAATACGA 50.0 45.0 58.0 57.2 648-657 bp 1.0 74.5